Optimization of Acridine Orange Staining for Buffalo Sperm, Cryopreserved in Egg Yolk based Extender to Detect DNA Fragmentation
نویسندگان
چکیده
منابع مشابه
P-137: Effect of Different Equilibration, Freezing and Thawing Rate on Cryopreserved Buffalo Spermatozoa Diluted inTris- Egg Yolk Extender
Background: Artificial insemination (AI) has been used for increasing genetic potential of animals in production while application of AI has been restricted in buffalo due to low freezing ability and fertility of frozen-thawed buffalo spermatozoa. So improvement in buffalo sperm cryopreservation may enhance the breeding programs of this valuable farm animal. Semen processing is one of the major...
متن کاملEffect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm
Cholesterol and Equex-STM are frequently added to different commercial and experimental extenders improving postthawing sperm quality. Doses of 125-150 mM of cholesterol from pig liver and 0.5-0.7% of Equex-STM were evaluated in a standard eggyolk extender (Martin et al., 1979). Six ejaculates per stallion from six pure Spanish stallions (6-8 years old) were collected in Martin's extender (B) a...
متن کاملEffects of egg yolk-citrate and milk extenders on chromatin structure and viability of cryopreserved bull sperm.
Semen from four Holstein bulls was evaluated to compare effects of four extender treatments on postthaw semen quality. Extender fractions A and B, either heated whole milk or 20% egg yolk-citrate, were combined to yield the extender treatments 1) milk and milk, 2) milk and egg yolk-citrate, 3) egg yolk-citrate and milk, and 4) egg yolk-citrate and egg yolk-citrate. Semen was evaluated at thawin...
متن کاملThe effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis?
OBJECTIVE The aim of this study was to determine whether Acridine Orange (AO) can be used as a routine procedure in a physician's office to detect sperm deoxyribonucleic acid (DNA) fragmentation in patients with varicoceles. MATERIAL AND METHODS Forty-five patients with a mean age of 30.4 years, who had been diagnosed with varicoceles by physical examination and 30 healthy males with a mean a...
متن کاملP-142: Effects of A Synthetic Antioxidant (4-Hydroxy Tempo) Additive to The Semen Extender on the Ejaculated Spermatozoa Characteristics before and after Freezing in Water Buffaloes (Bubalus Bubalis)
Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pakistan Journal of Zoology
سال: 2017
ISSN: 0030-9923
DOI: 10.17582/journal.pjz/2017.49.5.sc6